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All procedures, including animal care, wound modeling, dosing, and termination, were performed according to the Experimental Animal Guidelines for Ethical Review of Animal Welfare (GB/T 35892-2018) and approved by the Tianjin University Institute Animal Ethics Committee with the assigned approval number TJUE-2023-236
Basso DM, Beattie MS, Bresnahan JC
& Jonas, J.-C
After centrifugation, the pellets were resuspended in PBS, and the cell suspension was disrupted using an ultra-sonicator (Q125, Q-sonica, USA) in an ice bath for 15 min
Laboratory investigations may include: NF-B signaling TNF- expression IL-1 and IL-6 pathway analysis COX-2 regulation Oxidative stress mechanisms Cellular inflammatory responses These studies focus on understanding molecular signaling rather than evaluating therapeutic outcomes
GHK CID 73587
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