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Performance evaluation and quantitative analysis 4
Finally, adjust the polarizer until the difference in intensity values between the parallel and perpendiculr channels is maximized
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GHSR containing disks were separated from aggregates and possible trace amounts of the ligand through a size-exclusion chromatography step on a Superdex 200 increase 10/300 GL column (GE Healthcare) using a 25 mM Na-HEPES, 150 mM NaCl, 0.5 mM EDTA, pH 7.5 buffer as the eluent
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Lee WJ, Sim HB, Jang YH, et al
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