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The resin was then collected by centrifugation at 500 g for 10 min and washed in gravity flow column (Sangon Biotech) with five column volumes of buffer containing 20 mM HEPES (pH 7.5), 100 mM NaCl, 10% (v/v) glycerol, 5 mM MgCl2, 1 mM MnCl2, 25 M TCEP, 0.1% (w/v) LMNG, 0.02% (w/v) CHS, 2 M GLP-1 or LY3502970 and/or 10 M compound 2, followed by washing with fifteen column volumes of buffer containing 20 mM HEPES (pH 7.5), 100 mM NaCl, 10% (v/v) glycerol, 5 mM MgCl2, 1 mM MnCl2, 25 M TCEP, 0.03% (w/v) LMNG, 0.01% (w/v) glyco-diosgenin (GDN, Anatrace), 0.008% (w/v) CHS, 2 M GLP-1 or LY3502970 and/or 10 M compound 2
Without appropriate manufacturing oversight, theres no guarantee that the contents of the vial match the label
Glutathione injections should only be administered under medical supervision
Presented for research literature review only Nguyen HD et al
