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This idea dates back to seminal work from Eagle, who showed that supplementation with extracellular GSH supports cell growth in cystine-free conditions 22
The cell pellet was resuspended in ice-cold 100 mM Tris, pH 7.6, centrifuged again, and finally resuspended in 1 mL of the same buffer
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A deficiency can creep up gradually, making it easy to dismiss symptoms as stress or simply getting older. However, if left untreated, low B12 levels can lead to serious health issues
They are being negligent
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