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Small molecules from the human microbiota

GPX4, a seleno-protein, has seleno-cysteine as its active site (Yang et al., 2014

Because of this low oral bioavailability, clinical formulations of ALCAR must be dosed high enough to ensure that an adequate amount of the active compound survives the digestive process and reaches the blood-brain barrier

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Taking into account the K D = 34.7 fM ( K D determined from competition with His in the present work) , K D 1 = 3.7 10 9 M and K D 2 = 4.7 10 7 M values for Cu(II)-His complexes at pH 7.4 (taken from 31 ) it can be estimated that approximately 0.7 nM of Cu(II) are bound to His mainly in the form of CuHis 2 complex (Equations are presented in Materials and methods)