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The protein expression of the GCGR in the mouse cardiac regions cannot be readily tested at present because GCGR specific antibodies are not well characterized

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This might be due to unfavourable ionisation properties of the disulfide-containing peptide or the disulfide might negatively affect tryptic cleavage of the respective peptide before mass spectrometric analysis

For the analysis of the results the Kruskal-Wallys and Dunns Multiple Comparisons tests were realized, that revealed significant differences among the experimented groups