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Senescence-associated -galactosidase (SA--gal) staining SA--gal staining was performed according to the manufacturers instructions (#9860, Cell Signaling Technology)

ChIP assays were performed using the fast ChIP protocol with minor modifications

Lentiviral particles were re-suspended to a final concentration of 1.710 6 plaque formation untis (p.f.u.) per l

To meet the target for referral, practices will need to hit between 10-30% of patients aged 18 or over living with obesity, who have been referred to a weight management programme within 90 days of BMI being recorded

Presented for research literature review only Birkenfeld C et al