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The sections were immersed in phosphate-buffered saline containing 0.1% Triton X-100 (Nacalai Tesque) for permeabilization and incubated with rabbit polyclonal anti-phospho-histone H2A.X (H2AX) antibody (1:300
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The light subunit (molecular weight about 28 kDa) is catalytically inactive but plays an important regulatory role [12]
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