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RNA extraction and RT-PCR for gene expression analysis To determine the effect of HT stress on the transcription levels of Calvin cycle enzyme genes and antioxidant enzyme genes and their changes after GSH application, we tested photosynthetic genes including those encoding ribulose-1,5-bisphosphate carboxylase/oxygenase activase ( RCA ), RBCL and RBCS involved in CO 2 fixation
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A released pilot study following 6 patients, ahead of publication due to ongoing study, used markers of oxidative stress to evaluate GSH action from two dose forms: IV glutathione and oral Acetylglutathione over one week at a dose of 1400 mg
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