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Failure to do so will result in the return of the anemia and in the development of incapacitating and irreversible damage to the nerves of the spinal cord
Eryptosis: programmed death of nucleus-free, iron-filled blood cells
Usually less than one minute
For co-localization with transferrin and the endosomal marker EEA1, Alexa488 Halo-labeled cells were incubated with 5 g ml 1 Alexa568-transferrin (Tf568, Life technologies T23365) for 30 min in medium at 37 C, fixed and stained for EEA1 (Cell signaling 3288T) by immunofluorescence as described above, and imaged by Wide-field illumination microscopy on an OMX microscope and deconvolution
J Clin Hypertens (Greenwich)
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