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For cell proliferation assays with fold change in cell number, cells were cultured in replicates of 3 in 96-well plates at 1000 (suspension) or 150 (adherent) cells per well in 200 uL RPMI or DMEM base media under the conditions described in each experiment, and a separate group of 3 wells was also plated for each cell line with no treatment for an initial time point
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In rats, GCLC has a Km for glutamate that is about 10-fold higher, which is higher than the cellular glutamate concentration in most tissues
Studies evaluating GHK-Cus wound healing activity reported a 67% reduction in wound healing time compared to controls in preclinical models (Pickart, 1973)
N.et al (2004)
(2017) 181:805.e1
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