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Chromatographic separation of targeted analytes and internal standard was achieved on an Accucore C18 100 2.1 (2.6 particle size) column with a gradient elution of 10 mM of ammonium formate in water (phase A) and 10 mM of ammonium formate in methanol/2-propanol 80:20 (phase B) at a flow rate of 250 l/min

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172,173 For example, acid-sensitive imine linker (DAK) was conjugated with D-T7 peptide on the nanoparticle surface, which would break up in acidic environment, facilitating the endo/lysosomal escape

80 Cystine starvation has been reported to play an important role in cell death in different types of cancer