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The very low rate constants for direct, glutathione-independent dithiol-disulfide exchange reactions between glutaredoxins and roGFP2 or other protein(S 2 )/protein(SH) 2 couples (despite favorable thermodynamics) are a prerequisite and great advantage for reliable redox measurements in vivo
In order to further increase the production of GSH, the addition of amino acid precursors (5 mM glutamic acid, 5 mM cysteine and 5 mM glycine) was single shot after 36-h cultivation for feeding assay
The preparation pairs a pentadecapeptide derived from a gastric protein sequence with an acetylated heptapeptide corresponding to the actin-binding region of thymosin beta-4
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