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These portions underwent thermal denaturation for 5 min at precisely defined temperatures (37.0, 41.7, 45.8, 49.7, 53.5, 57.4, 61.3, 64.3, 67.0 C) using a MasterCycler EpGradient S thermal cycler (Eppendorf SE, DE)

Patients who continue losing weight through the first year often see continued incremental improvements, while those who plateau in weight typically plateau in lipid changes as well

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DPP4 has also been implicated as a novel marker of hypoxia inducible factor-1 (HIF-1) induction