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Glutathione: toxicological implications [PMID: 2188580] Laboratory literature summary: Glutathione: toxicological implications
Alcohol and Smoking Alcohol and smoking significantly increase oxidative stress, which can negate glutathione's antioxidant properties
Since the Apollo-NADP + sensor response depends on the interaction (dimerization) of exogenous mutant G6PD tagged with a fluorescent protein, interactions between transfected and endogenous G6PD may reduce sensor response
The samples were centrifuged, and the supernatant was subjected to a GSH assay based on a kinetic enzymatic recycling method that detects the oxidation of GSH by 5,5-dithiobis-2-nitrobenzoic acid (DTNB) and glutathione reductase to measure the GSH content in cells 74
Expected results Prior to the validation of our assay, we increased the expression of GSR by loading RAW264.7 Ms with oxidized low-density lipoprotein (oxLDL) and then stimulating with LPS
J., Hammond-Kosack, K
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