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& Baggiani, C
Although TR/GR-null livers generate some of their cysteine through the serine transsulfuration pathway, we here show that most cysteine in TR/GR-null livers comes from a pathway in which pyridoxal-phosphate-dependent cleavage of a carbonsulfur bond in cystine generates cysteine persulfide, which decomposes nonenzymatically into cysteine
20 g protein was separated by SDSPAGE (sodium dodecyl sulfatepolyacrylamide gel electrophoresis) on 8%, 10%, or 16% resolving gels (ThermoFisher Scientific, Waltham, MA, USA) and transferred to PVDF membrane (EMD Millipore, Burlington, MA, USA)
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Varadi M, Bertoni D, Magana P, et al
