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Assays for HCV RNA Qualitative Assays HCV RNA can be detected in the blood using amplification techniques such as polymerase chain reaction (PCR) or transcription- mediated amplification (TMA) 28

Pushing spatial resolution further, stimulated emission depletion (STED) nanoscopy utilizes a conventional excitation laser paired with a donut-shaped depletion laser, forcing peripheral fluorophores back to the ground state via stimulated emission and driving resolution down to 30-50 nm (204)

Individuals with a history of severe allergic reactions to injectable vitamins should be monitored closely or offered alternatives per clinician judgment

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Outcomes were assessed using the Childhood Autism Rating Scale (CARS) alongside measurements of free and total carnitine levels

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