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For melanoma that is not likely to spread to other parts of the body or recur, more tests may not be needed

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Beads were washed thrice with lysis buffer, twice with buffer A [50 mM Tris-HCl (pH 7.5), 100 mM NaCl, and 10% glycerol], and once with 50 mM Tris-HCl (pH 7.5)

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