For qRT-PCR, significance was calculated prior to transformation of C t values out of the log phase
Below I explain how approved oral formulations work, why many "drops" are controversial, and when an injectable makes more sense medically

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Reconstitution Quick Reference Community reconstitution protocols document the following dilution math: 10mg / 2mL = 5mg/mL = 5,000mcg/mL
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In addition, there is a particular effect on endothelial integrity (indicated as +2)