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After fixation, the cells were washed with PBS and permeabilized with 0.1% Triton X-100 (Daejung 8566-4405) in PBS for 10 min at room temperature

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2.8 CCK-8 assay HUVECs were evenly seeded into 96-well plates at a density of 1 10^4 cells per well, with a culture medium volume of 100 L per well

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