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As a measure of [Ca 2+ ] i , the fluorescence emission ratio at 340/380 excitation was calculated

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Univariate statistical testing using two-sample t-tests with confirmation by Wilcoxon rank-sum tests identified four metabolitescoumarin, L-leucine, L-octanoylcarnitine, and kaempferolthat differed significantly between ASD and control groups after FDR correction, with large effect sizes (Supplementary Table S2)