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Encapsulation of Nile red or fluorescein to PLGA nanoparticles To PLGA solution in acetone (1.0 mL, 25 mg/mL) either Nile red or PLGA-fluorescein was dissolved (0.2% w/w) and then the resulting solution was added drop wise into stirring water (5.0 mL)

Glutathione for skin whitening works by the following mechanisms: It neutralizes free radicals that can damage the skin, thus it gives out dull appearance and tends to make the skin look old before its time

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Furthermore, western blot analysis took place by initially preparing cell extracts in a lysis buffer followed by the standard method of ultrasonication

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