Sequencing was performed using a HiSeq 2500 (Illumina), with a read length of 300 bp paired-end reads and at least 25,000 tags per sample
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Individuals with risk factors for B12 deficiency including strict vegans, those with gastrointestinal conditions, patients taking long-term metformin or proton pump inhibitors, and older adultsshould discuss screening with their GP, even in the absence of obvious symptoms
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Tap the syringe to release air bubbles and then depress the plunger to remove all the remaining air and check to see that the dosage is correct