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Tandem mass spectrometry analysis of affinity-purified samples Three micrograms of each fraction or sample were auto-sampler loaded with an UltiMate 3000 HPLC pump onto a vented Acclaim Pepmap 100, 75 m 2 cm, nanoViper trap column (Thermo Fisher Scientific, Cat# 164535) coupled to a nanoViper analytical column (Thermo Fisher Scientific, Cat# 164570, 3 m, 100 , C18, 0.075 mm, 500 mm) with stainless steel emitter tip assembled on the Nanospray Flex Ion Source with a spray voltage of 2000 V

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Early recognition and appropriate management of side effects can often allow continued treatment with dose adjustments or supportive measures rather than requiring discontinuation of therapy

Testing is conducted by independent third-party laboratories including Janoshik

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Track symptoms and medication use