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The resultant complementary DNAs were eluted by mixing with 1 PCR reaction buffer and heating at 95 C for 5 min, followed by immediate separation of the supernatant from the beads

Kayser-Fleischer rings around the iris are considered pathognomic for Wilson disease when seen with acute liver failure, but they are seen in only about 50% of patients

Storage Instructions Lyophilised vials should be stored at -20C, protected from light and moisture, and remain stable for up to 24 months