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Nanoparticle-mediated brain drug delivery: overcoming bloodbrain barrier to treat neurodegenerative diseases

High-performance liquid chromatography (HPLC) was used to measure dopamine, metabolites of dopamine such as 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA), ascorbic acid, and dehydroascorbic acid in the striatal tissue

Ethical approval The seeds utilized in this study were obtained from the Indian Agriculture Research Institute (IARI) in New Delhi, which governs the seed manufacturing and processing

Storage: Store sealed in original packaging in a cool, dry place away from light

All mice were from a C57BL/6 background and were group-housed and maintained in individual ventilated cages with standard bedding and enrichment in a temperature- and humidity-controlled room on a 12-h light:dark cycle (lights on 7:00) with AL access to food (standard diet, Scientific Animal Food Engineering, or 45% HFD) and water unless otherwise stated

Dihexa Nasal Spray contains 10mg of Dihexa in a ready-to-use nasal spray formulation delivering approximately 100 sprays at 100mcg per spray