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Adenovirus delivery in proximal tubular cells To achieve the overexpression of GPX4 in proximal tubular cells, AAV9-mGPX4 or AAV9-vector at a dosage of 2.0 10 10 pfu was delivered into the mouse kidney (8-week-old C57BL/6 mice) by intraparenchymal injection 14 days before the UUO model was established

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Testing for subcutaneous depth