The proportion of p--syn in the normal state is very low, and an abnormal increase in p--syn induces the formation of pathological aggregates that contribute to neurotoxicity [10]
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For the extraction, methanol and internal standards were added, followed by incubation for 15 min at 30C on a thermal shaker (650 rpm, TS1 ThermoShaker, Biometra, Gttingen, Germany), centrifugation (21,000 x g, 10 min, 4C, Heraeus Fresco 21 Centrifuge, Thermo Scientific, Schwerte, Germany), evaporation, and centrifugation with methanol again
Control of neurosecretory vasopressin cells by noradrenergic projections of the caudal ventrolateral medulla
2023;doi:10.1210/clinem/dgad058