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Mann, J.B

GSH is synthesized from three amino acids in two ATP-dependent enzymatic steps

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Solid-phase peptide synthesis of D-amino acid chains of this length requires specialized reagents (D-Fmoc amino acids are significantly more expensive than their L-counterparts), extended synthesis times, and careful quality control to ensure correct sequence and sufficient purity

The remaining cells were washed three times with PBS and transferred to 6-well plates containing cystine-free medium supplemented with unlabelled GSH