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Total RNA was isolated by using TRIzol reagent, treated with DNase I, and converted to cDNA as previously described 66

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[1] [4] While this appetite suppression facilitates substantial weight loss, it also creates a clinical challenge: ensuring adequate protein intake to preserve lean body mass

doi: 10.2337/dc07-0228 38 DeaconCFHughesTEHolstJJ