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Biomolecules (2021) 13
Immunoblotting Proteins were extracted from cultured SCD erythroblasts or spleen Ter119+ erythroblasts with modified RIPA buffer (150 mM NaCl, 50 mM Tris, pH 7.5, 1% NP40, 0.25% Na-deoxycholate) plus protease inhibitor cocktail (Sigma) and stored at 80 C up to processing
(PubMed) Lei YP, Chen HW, Sheen LY, Lii CK
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Usage is often described as AM and PM , depending on how the rest of the routine is built and how sensitive the skin is
Ostergaard et al., 2000)
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