Insert the needle into the vial without pushing or pulling the plunger
Following 3 hours of feeding the CR mice at the start of the light cycle, all dietary groups underwent a 3 hr fast, followed by cervical dislocation and tissues for molecular analysis were flash-frozen in liquid nitrogen or fixed and prepared as described in the methods below
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In humans, alpha, mu, pi and sigma isoenzymes contain a tyrosine in the G-site while the other classes a serine or a cysteine
10.1073/pnas.1607152113 Proc