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Add the clear sample containing target GST-tagged protein onto the equilibrated gravity column, keep the sample in column for at least 2 min, ensure that the sample fully binds with the resin, and collect the flowthrough with a flow-rate of 0.5 - 1 mL per minute, then add the flowthrough onto the column again, repeat the previous operation, this can increase the binding efficiency
It should be noted, however, that resistance and HR (programmed cell death) do not necessarily correlate (Bendahmane et al., 2016)
- Noor R
Out of sunlight
4.2 Lycium ruthenicum Lycium ruthenicum, commonly known as black wolfberry, is a perennial shrub belonging to the Solanaceae family
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