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Funny story I actually went to the supermarket with my mother-in-law and bought some 70% absinthe because it was the only drinkable alcohol I could find over 50% here in Spain
Mosquito supernatants containing both hemolymph and cuticle dopamine were obtained by adding 200 l ice-cold 0.2 M perchloric acid to each tube, homogenizing for 20 s on ice, and centrifuging at 20,000 g for 10 min at 4 C 127
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Importantly, food intake did not change, confirming that the mechanism was direct fat mobilization rather than appetite suppression (Heffernan et al., 2001)
The observed effect was least pronounced in the BSO-treated group, as shown in western blot
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