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Immunoprecipitation Hepatocytes were lysed in an immunoprecipitation (IP) buffer (150 mM NaCl), 50 mM Tris-HCl, 1% Nonidet P-40, pH 7.8, and a mammalian cell-specific protease inhibitor cocktail
(PubMed) Hatono S, Jimenez A, Wargovich MJ
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Endothelial progenitor cells: characterization, pathophysiology, and possible clinical relevance
For details, see the Semaglutide precautions section
Briefly, we dissolved the powder OA (O-1008 Sigma-Aldrich, Germany) at a final concentration of 12 mM in phosphate-buffered saline (PBS
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