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Rosenfeld WE, Doose DR, Walker SA

RNA extraction and reverse transcription The collected plant tissues were cleaned three times with deionized water, rapidly frozen in liquid nitrogen, and then stored in a 80 C refrigerator for RNA extraction

The Smad2/3Smad4 complex then translocates into the nucleus to regulate transcription

Zinc supports physical barriers

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Utilizing a forward biochemical screening approach, the researchers successfully reconstituted cGLR signaling from representatives across the protein family tree and discovered 16 active animal cGLRs