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Like the Ov GST1, FhGST-S1 does not have this Trp residue and therefore has a longer 4, although the FhGST-S1 helix aligns better to that of the mammalian PGDSs (Fig
)
Sterile water lacks the bacteriostatic agent, meaning bacteria can begin to grow after the first use, contaminating the entire vial
GHK-Cu is not approved by the FDA for systemic therapeutic use (injection) as a drug
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A practical consequence of this coordination chemistry: any in vitro experiment that adds free GHK to cell culture medium containing serum (and therefore copper bound to albumin and other carrier proteins) is effectively delivering a mixture of free GHK and GHK-Cu, with the equilibrium dependent on copper availability
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