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M., Wu, Y., & Hazen, S

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The diet was tailored to provide 55% carbohydrates, 30% fat, and 15% protein, with a 300-kilocalorie deficit based on individual energy requirements calculated using the Harris-Benedict formula 17

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After incubation for 10 min, 200 L luciferin solution (containing 20 mM tricine, 1.0 mM MgSO4, 0.1 mM EDTA, 33 mM dithiothreitol, 270 M coenzyme A, 500 M D-luciferin, and 530 M ATP, pH 7.8) was added, and then the firefly luminescence was measured with a BioTek synergy 4 Multi-Mode Microplate Reader