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This threshold is highly consistent with the basic mechanism of action of tDCS: Nitsche and Paulus confirmed that anodal tDCS requires at least 13 min to induce long-term potentiation-like effects through NMDA receptor-dependent synaptic plasticity mechanisms, and the subsequent effects can last for 30 to 120 min (Nitsche and Paulus, 2000
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10.1093/jxb/erv063 J
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After reconstitution, it must remain refrigerated and used within the timeframe specified by the manufacturer
