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Activation of nicotine receptors on oral melanocytes by acetylcholine or by nicotine may bring about melanin hyperpigmentation [4, 6, 30]

The results of Beggs test revealed no presence of a small-study effect ( P = 0806)

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NMR chemical shift perturbation analysis The CSPs were calculated according to the following equation: where H and N indicate the chemical shift changes of the amide proton and nitrogen, respectively, using CcpNMR 2.5 49