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Wash unbound components and add HRP-Streptavidin Conjugate (SABC)
However, there is no evidence that enforcing reduction of these sensors is sufficient for their activation, meaning there would then be no reason to expect DCA to cause ER stress on its own
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In regions such as the African and Asian continents and the Mediterranean river basin there exists a high frequency of the different variants of the enzymatic deficiency, whereas in China and Japan the frequency is low 23
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