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Luciferase readings were measured using a dual-luciferase reagent kit (Promega) according to the manufacturers protocol

c, unpaired t-test

doi: 10.21037/tau.2017.09.13 66 AgarwalALeisegangKMajzoubAHenkelRFinelliRSelvamMKPet al

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Statistical significance was assessed using two-tailed unpaired t-tests with false discovery rate (FDR) correction using the two-stage step-up method of Benjamini, Krieger and Yekutieli (Q = 1%)

Potential theoretical advantages include: Multi-pathway appetite suppression combining amylin and incretin signals Enhanced metabolic flexibility through glucagon receptor activation (retatrutide) and amylin-mediated effects (cagrilintide) Complementary gastric emptying effects from both compounds Broader receptor coverage addressing multiple aspects of energy homeostasis However, it's critical to emphasize: no published clinical trials have evaluated the cagrilintide dosage with retatrutide combination as of 2026