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This represents a failure in the elimination phase of immunoediting, allowing tumors to escape initial immune recognition by disrupting neoantigen presentation and preventing the activation of nave T cells
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Cells were cultured at 37 C in a humidified atmosphere of 5% CO 2 with medium changed every 2 days until confluence
Huh7, Hep3B, and HepG2 were maintained in DMEM (ThermoFisher Scientific) supplemented with 10% FBS (Hyclone)
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