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If the vial can hold 5 mL, that water volume halves the concentration from 32 mg/mL to 16 mg/mL and may reduce the copper-peptide sting

Smalheiser et al

Unlike standard sterile water, which must be discarded immediately after the seal is broken, bacteriostatic water can be drawn from over the course of approximately 28 days when refrigerated making it the practical choice for research workflows that require multiple draws from the same vial

For those who have struggled with maintaining adequate Vitamin D levels through diet and sun exposure alone, injections provide an effective alternative