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Post-treatment, the cells were washed with 1X phosphate-buffered saline and stained with 15 M DCFDA-H2 or 5 M MitoSOX for 15 min at 37 C
Biochem Med (Zagreb) 2014
The 2006 Ionescu paper documented that pulsatile GH secretion persists even under continuous receptor stimulation, suggesting somatotroph-intrinsic pulsatile release dynamics independent of receptor-binding temporal patterns (PMID 17018654)
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