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In auto MS/MS acquisition, the instrument was set to acquire over the m/z range 25-1000 Da, and the accumulation time for product ion scan was set at 0.05 s/spectra
How it Works Vitamin B12, a water-soluble vitamin that is essential for many bodily processes, including the production of red blood cells and the functioning of the nervous system, is delivered directly into the bloodstream
Q&A: insulin secretion and type 2 diabetes: why do -cells fail
The recycling reaction was initiated by the addition of reaction buffer (100 mM sodium phosphate, pH 7.4), supplemented with 5,5-dithiobis (2-nitrobenzoic acid) (DTNB) (100 M) (Sigma), NADPH (100 M) (Roth), glutathione reductase (1 U/ml) (Sigma), and EDTA (1 mM) (Sigma)
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