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For colony formation assays, cells (5 10 4 ) were suspended in 2 mL of 0.33% noble agar (Difco) and laid over 6-well culture plates previously coated with 2 mL of 0.5% noble agar
Ebben JD, Treisman DM, Zorniak M, Kutty RG, Clark PA, Kuo JS
All participants provided written informed consent, and the study was approved by the Medical Ethics Committees of all the participating universities
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These effects are usually temporary
it can invalidate the question the protocol was designed to answer
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