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Cell lines and cell culture Mouse tracheal epithelial cells (MTECs) were obtained from normal tracheal tissue of 3-week-old Gprc5a +/+ and Gprc5a / mice (C57BL/6 X129sv) as described [27, 28]

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A practical framework includes: Use the lowest protocol complexity that answers the question: If one schedule can test the hypothesis, adding extra administrations only adds interpretive burden

Tkalcevic et al., 2007