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Lysate samples were obtained by adding 250 l lysis buffer to each well, scraping and collecting cellular contents, followed by centrifugation of collected lysates at 8000 g for 10 min at 4 C

Research Applications In laboratory research, AMINO-1MQ is investigated for NNMT enzyme inhibition and its downstream effects on NAD+ and S-adenosylmethionine (SAM) metabolism, adipocyte lipogenesis, and energy-expenditure pathways

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These are generally mild and tend to resolve on their own

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