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"The 'Skinny' on brown fat, obesity, and bone." American Journal of Biological Anthropology , November 2014, Fielding, Roger et al

Participants in both the Tirzepatide and Semaglutide treatment arms received lifestyle intervention, including a reduced-calorie diet and increased physical activity

The viability of HT22 cells following A 142 stimulation was further assessed using live/dead cell staining and Cell Counting Kit8 (CCK8) assays (Beyotime, China)

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These assessments included immunostaining for 8OHG, IBA1, and A plaques, as well as immunohistochemistry for ptau and Nissl staining for the evaluation of neuropathological changes

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